---
id: "claim:a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex-20-b4e8dc3922:2b75269c5ebf698f8e16"
kind: "claim"
title: "Independent open-chromatin analysis identified 33 cell types."
description: ""
url: "https://sina.bio/claim/independent-open-chromatin-analysis-identified-33-cell-types"
---

# Independent open-chromatin analysis identified 33 cell types.

## Content

Independent open-chromatin analysis identified 33 cell types.

## Metadata

```json
{
  "claim_position": 55,
  "source_publication_id": "version:a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex--2021-10-06-nature",
  "source_work_id": "work:a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex"
}
```

## Connections (3)

### A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex

- ID: `version:a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex--2021-10-06-nature`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex>

This publication contains this grounded claim.

> n=33celltypesusingopenchromatin

Source: 2021-10-06-nature.pdf — <https://sina.bio/source/2021-10-06-nature-pdf-573f17d0>

### Sequencing open chromatin of single cell nuclei: snATAC-seq v1

- ID: `publication:doi:10.17504/protocols.io.pjudknw`
- Kind: `publication`
- Direction: `outgoing`
- URL: <https://sina.bio/publication/sequencing-open-chromatin-of-single-cell-nuclei-snatac-seq-v1>

This publication is cited in support of this claim.

> Combiningtranscriptomesandepigenomes
> RegionsofopenchromatinandpatternsofDNAmethylation,includ-
> ingCGandnon-CGmethylation,arecell-type-specificsignaturesof
> neuronalidentityandcanbeassayedinsinglenuclei9,11
> .Weapplied
> snmC-seq2(ref.14
> )(9,876cells)andsnATAC-seq28
> (81,196cells)assays
> tonucleiisolatedfromthesameMOpsamples.Independentanaly-
> sesofeachepigenomicdatasetidentifiedn=42celltypesusingDNA
> methylation,andn=33celltypesusingopenchromatin(ExtendedData
> Fig.6a–d,SupplementaryTable4).Markergenesformajorcellclasses
> hadcorrespondingpatternsofcell-type-specificdepletionofnon-CG
> methylation(lowmCH;ExtendedDataFig.6b)andopenchromatinin
> thegenebody(ExtendedDataFig.6d).

Source: 2021-10-06-nature.pdf — <https://sina.bio/source/2021-10-06-nature-pdf-573f17d0>

### Single-nucleus methylation and open-chromatin assays identified cell-type-specific neuronal identity signatures that ali

- ID: `result:a-transcriptomic-and-epigenomic-cell-atlas-of-the-mouse-primary-motor-cortex-20-b4e8dc3922:032e34cd82cc7bd63cd0`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/single-nucleus-methylation-and-open-chromatin-assays-identified-cell-ty-fa9a4d1a>

This claim supports this result.