---
id: "claim:benchmarking-of-lightweight-mapping-based-single-cell-rna-seq-pre-processing-20-649c7d9c44:8f3fade945fc3b36323a"
kind: "claim"
title: "BUS files decouple technology-dependent pseudoalignment from barcode correction, UMI collapsing, and read counting."
description: ""
url: "https://sina.bio/claim/bus-files-decouple-technology-dependent-pseudoalignment-from-barcode-co-dd0bfaf5"
---

# BUS files decouple technology-dependent pseudoalignment from barcode correction, UMI collapsing, and read counting.

## Content

BUS files decouple technology-dependent pseudoalignment from barcode correction, UMI collapsing, and read counting.

## Metadata

```json
{
  "claim_position": 25,
  "source_publication_id": "version:benchmarking-of-lightweight-mapping-based-single-cell-rna-seq-pre-processing--2021-03-03-biorxiv",
  "source_work_id": "work:benchmarking-of-lightweight-mapping-based-single-cell-rna-seq-pre-processing"
}
```

## Connections (1)

### Benchmarking of lightweight-mapping based single-cell RNA-seq pre-processing

- ID: `version:benchmarking-of-lightweight-mapping-based-single-cell-rna-seq-pre-processing--2021-03-03-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/benchmarking-of-lightweight-mapping-based-single-cell-rna-seq-pre-processing>

This publication contains this grounded claim.

> This serves to decouple the technology dependent pseudoalignment step from the other single-cell RNA-seq pre-processing steps such as barcode error correction, unique molecular identifier collapsing and read counting.

Source: 2021-03-03-biorxiv.pdf — <https://sina.bio/source/2021-03-03-biorxiv-pdf>