---
id: "claim:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:29e8daff73b40b1c1e98"
kind: "claim"
title: "kb ref needs to be run only once for a reusable mapping index."
description: ""
url: "https://sina.bio/claim/kb-ref-needs-to-be-run-only-once-for-a-reusable-mapping-index"
---

# kb ref needs to be run only once for a reusable mapping index.

## Content

kb ref needs to be run only once for a reusable mapping index.

## Metadata

```json
{
  "claim_position": 115,
  "source_publication_id": "version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv",
  "source_work_id": "work:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq"
}
```

## Connections (2)

### kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

- ID: `version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-si-70b58d03>

This publication contains this grounded claim.

> The kb ref command only needs to be run once to create the index against which reads will be mapped.

Source: 2023-11-22-biorxiv.pdf — <https://sina.bio/source/2023-11-22-biorxiv-pdf>

### A reusable mouse reference index was built in about 15 minutes for standard targets and one hour for nascent-plus-mature

- ID: `result:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:72685d4a1739390372e7`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/a-reusable-mouse-reference-index-was-built-in-about-15-minutes-for-stan-486c2347>

This claim supports this result.