---
id: "claim:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:65f951fe75f0d2cb1e2d"
kind: "claim"
title: "Assays with variable barcode or UMI positions require FASTQ preprocessing into a supported fixed structure."
description: ""
url: "https://sina.bio/claim/assays-with-variable-barcode-or-umi-positions-require-fastq-preprocessi-8c016cb3"
---

# Assays with variable barcode or UMI positions require FASTQ preprocessing into a supported fixed structure.

## Content

Assays with variable barcode or UMI positions require FASTQ preprocessing into a supported fixed structure.

## Metadata

```json
{
  "claim_position": 62,
  "source_publication_id": "version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv",
  "source_work_id": "work:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq"
}
```

## Connections (1)

### kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

- ID: `version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-si-70b58d03>

This publication contains this grounded claim.

> due to barcodes or UMIs of variable lengths and positions), preprocessing of the FASTQ file should be performed beforehand to reformat the reads into a structure that can be handled by this format.

Source: 2023-11-22-biorxiv.pdf — <https://sina.bio/source/2023-11-22-biorxiv-pdf>