---
id: "claim:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:d430215ad0f2d53531d2"
kind: "claim"
title: "Generating a transcriptome during indexing keeps the transcriptome, index, FASTA, and GTF mutually consistent."
description: ""
url: "https://sina.bio/claim/generating-a-transcriptome-during-indexing-keeps-the-transcriptome-inde-3066f919"
---

# Generating a transcriptome during indexing keeps the transcriptome, index, FASTA, and GTF mutually consistent.

## Content

Generating a transcriptome during indexing keeps the transcriptome, index, FASTA, and GTF mutually consistent.

## Metadata

```json
{
  "claim_position": 36,
  "source_publication_id": "version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv",
  "source_work_id": "work:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq"
}
```

## Connections (1)

### kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

- ID: `version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-si-70b58d03>

This publication contains this grounded claim.

> Indexing with kb-python has the advantage that a reference transcriptome is generated directly from a FASTA and GTF ensuring consistency between the transcriptome reference, its associated index, and the input FASTA and GTF.

Source: 2023-11-22-biorxiv.pdf — <https://sina.bio/source/2023-11-22-biorxiv-pdf>