---
id: "claim:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:fa8f1bf54c81767330bf"
kind: "claim"
title: "kallisto introduced pseudoalignment to improve mapping accuracy while reducing runtime and memory use for bulk RNA-seq p"
description: "kallisto introduced pseudoalignment to improve mapping accuracy while reducing runtime and memory use for bulk RNA-seq preprocessing."
url: "https://sina.bio/claim/kallisto-introduced-pseudoalignment-to-improve-mapping-accuracy-while-r-b6e95b95"
---

# kallisto introduced pseudoalignment to improve mapping accuracy while reducing runtime and memory use for bulk RNA-seq p

kallisto introduced pseudoalignment to improve mapping accuracy while reducing runtime and memory use for bulk RNA-seq preprocessing.

## Content

kallisto introduced pseudoalignment to improve mapping accuracy while reducing runtime and memory use for bulk RNA-seq preprocessing.

## Metadata

```json
{
  "claim_position": 8,
  "source_publication_id": "version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv",
  "source_work_id": "work:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq"
}
```

## Connections (2)

### kallisto, bustools and kb-python for quantifying bulk, single-cell and single-nucleus RNA-seq

- ID: `version:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nucleus-rna-seq--2023-11-22-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-si-70b58d03>

This publication contains this grounded claim.

> 1,15–21 kallisto8 introduced the pseudoalignment paradigm for improving the accuracy of alignment and reducing runtimes and memory footprint of bulk RNA-seq preprocessing and, with the development of bustools22 , has been adapted for both single-cell RNA-seq quantification1 and single-nucleus RNA-seq quantification.

Source: 2023-11-22-biorxiv.pdf — <https://sina.bio/source/2023-11-22-biorxiv-pdf>

### Pseudoalignment combines accurate read assignment with low runtime and memory use through a colored de Bruijn graph inde

- ID: `result:kallisto-bustools-and-kb-python-for-quantifying-bulk-single-cell-and-single-nuc-d2d227835f:ef80e876b49d2988a330`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/pseudoalignment-combines-accurate-read-assignment-with-low-runtime-and-e7260a99>

This claim supports this result.