---
id: "claim:long-read-sequencing-transcriptome-quantification-with-lr-kallisto-2025-12-01-p-c105976de1:090d49bbc38711b1447d"
kind: "claim"
title: "Higher UMI depth per nucleus yielded higher ONT-versus-Illumina Spearman correlations."
description: ""
url: "https://sina.bio/claim/higher-umi-depth-per-nucleus-yielded-higher-ont-versus-illumina-spearma-06595f0d"
---

# Higher UMI depth per nucleus yielded higher ONT-versus-Illumina Spearman correlations.

## Content

Higher UMI depth per nucleus yielded higher ONT-versus-Illumina Spearman correlations.

## Metadata

```json
{
  "claim_position": 70,
  "source_publication_id": "version:long-read-sequencing-transcriptome-quantification-with-lr-kallisto--2025-12-01-plos-computational-biology",
  "source_work_id": "work:long-read-sequencing-transcriptome-quantification-with-lr-kallisto"
}
```

## Connections (2)

### Long-read sequencing transcriptome quantification with lr-kallisto

- ID: `version:long-read-sequencing-transcriptome-quantification-with-lr-kallisto--2025-12-01-plos-computational-biology`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/long-read-sequencing-transcriptome-quantification-with-lr-kallisto-3ccc217c>

This publication contains this grounded claim.

> Increased UMI depth per nucleus yields higher Spearman correlations, and given that the libraries were not fully saturated by sequencing (when downsampling the ONT reads by half, we found only 803721 out of 946087 bar- codes, a reduction of 15%), it seems likely that with increased sequencing depth short and long read correlations may improve (Fig BivI in S1 Text).

Source: 2025-12-01-plos-computational-biology.pdf — <https://sina.bio/source/2025-12-01-plos-computational-biology-pdf>

### Single-nucleus ONT quantification recovered barcodes also found in Illumina reads, and greater ONT sequencing and UMI de

- ID: `result:long-read-sequencing-transcriptome-quantification-with-lr-kallisto-2025-12-01-p-c105976de1:6efc4e06c971bc7f9464`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/single-nucleus-ont-quantification-recovered-barcodes-also-found-in-illu-4800c5a0>

This claim supports this result.