---
id: "claim:modular-efficient-and-constant-memory-single-cell-rna-seq-preprocessing-2021-04-60a02544e2:29d61828528e22765fa9"
kind: "claim"
title: "Distinct observed UMIs per gene and inter-gene collisions were used to estimate intra-gene collisions."
description: ""
url: "https://sina.bio/claim/distinct-observed-umis-per-gene-and-inter-gene-collisions-were-used-to-9a68dda7"
---

# Distinct observed UMIs per gene and inter-gene collisions were used to estimate intra-gene collisions.

## Content

Distinct observed UMIs per gene and inter-gene collisions were used to estimate intra-gene collisions.

## Metadata

```json
{
  "claim_position": 88,
  "source_publication_id": "version:modular-efficient-and-constant-memory-single-cell-rna-seq-preprocessing--2021-04-01-nature-biotechnology",
  "source_work_id": "work:modular-efficient-and-constant-memory-single-cell-rna-seq-preprocessing"
}
```

## Connections (1)

### Modular, efficient and constant-memory single-cell RNA-seq preprocessing

- ID: `version:modular-efficient-and-constant-memory-single-cell-rna-seq-preprocessing--2021-04-01-nature-biotechnology`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/modular-efficient-and-constant-memory-single-cell-rna-seq-preprocessing>

This publication contains this grounded claim.

> UMI collision estimates. A UMI associated with a read from a cell is said to have 'collided' if it appears in two or more reads originating from different molecules. To estimate UMI collision rates, two types of information were used. First, reads in the same cell that originate from different genes must have originated from different molecules, and therefore the sharing of a UMI between two such reads was used as an indicator of a collision. Second, for each gene, the number of distinct UMIs associated within it was measured from the data. Based on the assumption that UMIs were sampled uniformly at random from beads, these data were used to estimate the number of intra-gene collisions (see Supplementary Note). The assumption was verified by examining the distribution of UMI counts across cells; the empirical distribution was near uniform with the exception of a handful of UMIs (Supplementary Note Fig. 2).

Source: 2021-04-01-nature-biotechnology.pdf — <https://sina.bio/source/2021-04-01-nature-biotechnology-pdf>