---
id: "claim:quantifying-orthogonal-barcodes-for-sequence-census-assays-2022-10-10-biorxiv:521acf04b6b22c361857"
kind: "claim"
title: "BC49 and BC74 are 8 bp long and share subsequences up to 7 bp."
description: ""
url: "https://sina.bio/claim/bc49-and-bc74-are-8-bp-long-and-share-subsequences-up-to-7-bp-c5609234"
---

# BC49 and BC74 are 8 bp long and share subsequences up to 7 bp.

## Content

BC49 and BC74 are 8 bp long and share subsequences up to 7 bp.

## Metadata

```json
{
  "claim_position": 81,
  "source_publication_id": "version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2022-10-10-biorxiv",
  "source_work_id": "work:quantifying-orthogonal-barcodes-for-sequence-census-assays"
}
```

## Connections (2)

### Quantifying orthogonal barcodes for sequence census assays

- ID: `version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2022-10-10-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/quantifying-orthogonal-barcodes-for-sequence-census-assays-cc9d35e3>

This publication contains this grounded claim.

> We observed slight discordance with two feature barcodes, BC49 and BC74, when processing the PDX samples assayed with Multiseq. These two Multiseq barcodes are short (8 bp in length), yet share long subsequences up to 7 bp in length (Supplementary Figure 4a). The introduction of errors during sequencing can result in possible incorrect barcode assignment and loss of barcodes (Methods: Barcode simulation). We addressed this issue by trimming the excess sequence surrounding the 8 bp feature barcode (Methods: Multiseq).

Source: 2022-10-10-biorxiv.pdf — <https://sina.bio/source/2022-10-10-biorxiv-pdf>

### Short shared Multiseq barcode subsequences caused quantification discordance that was addressed by trimming excess seque

- ID: `result:quantifying-orthogonal-barcodes-for-sequence-census-assays-2022-10-10-biorxiv:2f9233336db2d7c6aec8`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/short-shared-multiseq-barcode-subsequences-caused-quantification-discor-7cabdb66>

This claim supports this result.