--- id: "claim:quantifying-orthogonal-barcodes-for-sequence-census-assays-2022-10-10-biorxiv:8f628f0a5ad07feea847" kind: "claim" title: "kITE assignments were fully discordant for 1.6% of TAP-Seq cells." description: "" url: "https://sina.bio/claim/kite-assignments-were-fully-discordant-for-1-6-of-tap-seq-cells" --- # kITE assignments were fully discordant for 1.6% of TAP-Seq cells. ## Content kITE assignments were fully discordant for 1.6% of TAP-Seq cells. ## Metadata ```json { "claim_position": 96, "source_publication_id": "version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2022-10-10-biorxiv", "source_work_id": "work:quantifying-orthogonal-barcodes-for-sequence-census-assays" } ``` ## Connections (2) ### Quantifying orthogonal barcodes for sequence census assays - ID: `version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2022-10-10-biorxiv` - Kind: `publication` - Direction: `incoming` - URL: This publication contains this grounded claim. > We also processed 10,860 K562 cells expressing dCas9–KRAB from TAP-Seq transduced with 86 sgRNAs targeting 14 promoters or enhancers and 30 control sgRNAs to compare assignment of sgRNAs to individual cells. We found 97.8% of all 10,860 barcodes are fully concordant with TAP-Seq assignments, 0.53% of cells are partially concordant, and 1.6% of cells are fully discordant. (Methods: TAP-Seq). The discrepancies are likely the result of differences between mapping procedures producing differences in counts near the threshold (8 umi counts) resulting in differential cell assignment for cells with low UMI counts (Supplementary Figure 6). Source: 2022-10-10-biorxiv.pdf — ### kITE assignments were highly concordant with TAP-Seq assignments, with 97.8% full concordance, 0.53% partial concordance - ID: `result:quantifying-orthogonal-barcodes-for-sequence-census-assays-2022-10-10-biorxiv:3a7896637900d72c0a02` - Kind: `result` - Direction: `incoming` - URL: This claim supports this result.