---
id: "claim:quantifying-orthogonal-barcodes-for-sequence-census-assays-2024-01-05-bioinform-db2d59bea4:37d3bf36c721594e03fd"
kind: "claim"
title: "10x CRISPR reads were trimmed to retain the 19-bp barcode plus set overhang."
description: ""
url: "https://sina.bio/claim/10x-crispr-reads-were-trimmed-to-retain-the-19-bp-barcode-plus-set-over-3a8720fc"
---

# 10x CRISPR reads were trimmed to retain the 19-bp barcode plus set overhang.

## Content

10x CRISPR reads were trimmed to retain the 19-bp barcode plus set overhang.

## Metadata

```json
{
  "claim_position": 97,
  "source_publication_id": "version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2024-01-05-bioinformatics-advances",
  "source_work_id": "work:quantifying-orthogonal-barcodes-for-sequence-census-assays"
}
```

## Connections (1)

### Quantifying orthogonal barcodes for sequence census assays

- ID: `version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2024-01-05-bioinformatics-advances`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/quantifying-orthogonal-barcodes-for-sequence-census-assays-f4f3e077>

This publication contains this grounded claim.

> The FASTQ reads were trimmed using seqtk trimfq with
> the -e option set to the length of the read minus the 19 plus
> overhang such that only the 19-bp barcode plus the set over
> hang was retained.

Source: 2024-01-05-bioinformatics-advances.pdf — <https://sina.bio/source/2024-01-05-bioinformatics-advances-pdf>