---
id: "claim:quantifying-orthogonal-barcodes-for-sequence-census-assays-2024-01-05-bioinform-db2d59bea4:bcd8198dda5debf7029c"
kind: "claim"
title: "A mutation two bases before EZR-1 can make a read ambiguous."
description: ""
url: "https://sina.bio/claim/a-mutation-two-bases-before-ezr-1-can-make-a-read-ambiguous-828011a0"
---

# A mutation two bases before EZR-1 can make a read ambiguous.

## Content

A mutation two bases before EZR-1 can make a read ambiguous.

## Metadata

```json
{
  "claim_position": 43,
  "source_publication_id": "version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2024-01-05-bioinformatics-advances",
  "source_work_id": "work:quantifying-orthogonal-barcodes-for-sequence-census-assays"
}
```

## Connections (2)

### Quantifying orthogonal barcodes for sequence census assays

- ID: `version:quantifying-orthogonal-barcodes-for-sequence-census-assays--2024-01-05-bioinformatics-advances`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/quantifying-orthogonal-barcodes-for-sequence-census-assays-f4f3e077>

This publication contains this grounded claim.

> Therefore, a single mutation
> two bases away from the start of the EZR-1 sgRNA sequence
> could result in an ambiguous read.

Source: 2024-01-05-bioinformatics-advances.pdf — <https://sina.bio/source/2024-01-05-bioinformatics-advances-pdf>

### The largest kITE-Cell Ranger CRISPR discrepancies involved guides sharing 16-bp subsequences; a nearby mutation could ma

- ID: `result:quantifying-orthogonal-barcodes-for-sequence-census-assays-2024-01-05-bioinform-db2d59bea4:b8e700af7643b95ec2a8`
- Kind: `result`
- Direction: `incoming`
- URL: <https://sina.bio/result/the-largest-kite-cell-ranger-crispr-discrepancies-involved-guides-shari-9958f479>

This claim supports this result.