--- id: "claim:reliable-and-accurate-diagnostics-from-highly-multiplexed-sequencing-assays-202-1913269985:4572194cebdd3a96d77c" kind: "claim" title: "By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that wh" description: "By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that when the sample sheet used for demultiplexing has two index barcodes that are Hamming distance n apart, bcl2fastq exists with an error when the Hamming distance used for error correction is less than n/2+1." url: "https://sina.bio/claim/by-generating-bcl-files-from-a-set-of-fastq-records-and-running-the-bcl-7b9c91b4" --- # By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that wh By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that when the sample sheet used for demultiplexing has two index barcodes that are Hamming distance n apart, bcl2fastq exists with an error when the Hamming distance used for error correction is less than n/2+1. ## Content By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that when the sample sheet used for demultiplexing has two index barcodes that are Hamming distance n apart, bcl2fastq exists with an error when the Hamming distance used for error correction is less than n/2+1. ## Metadata ```json { "claim_position": 79, "source_publication_id": "version:reliable-and-accurate-diagnostics-from-highly-multiplexed-sequencing-assays--2020-12-10-nature-scientific-reports", "source_work_id": "work:reliable-and-accurate-diagnostics-from-highly-multiplexed-sequencing-assays" } ``` ## Connections (2) ### Reliable and accurate diagnostics from highly multiplexed sequencing assays - ID: `version:reliable-and-accurate-diagnostics-from-highly-multiplexed-sequencing-assays--2020-12-10-nature-scientific-reports` - Kind: `publication` - Direction: `incoming` - URL: This publication contains this grounded claim. > By generating BCL files from a set of FASTQ records and running the BCL files through bcl2fastq (code), we found that when the sample sheet used for demultiplexing has two index barcodes that are Hamming distance n apart, bcl2fastq exists with an error when the Hamming distance used for error correction is less than n/2+1. Source: 2020-12-10-nature-scientific-reports.pdf — ### bcl2fastq sample-index correction can show unwanted behavior because correction thresholds depend on barcode distance an - ID: `result:reliable-and-accurate-diagnostics-from-highly-multiplexed-sequencing-assays-202-1913269985:74efc40eb4b95098d81e` - Kind: `result` - Direction: `incoming` - URL: This claim supports this result.