---
id: "claim:single-nucleus-analysis-of-human-white-adipose-tissue-reveals-adipocyte-subsets-c9bd0e4584:a1e2fc30a3db76ea224c"
kind: "claim"
title: "Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to "
description: "Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to the expression of the housekeeping gene were calculated using the ∆∆Ct method."
url: "https://sina.bio/claim/gene-expression-analysis-utilized-quantstudio-software-applied-biosyste-e5508a84"
---

# Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to

Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to the expression of the housekeeping gene were calculated using the ∆∆Ct method.

## Content

Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to the expression of the housekeeping gene were calculated using the ∆∆Ct method.

## Metadata

```json
{
  "claim_position": 321,
  "source_publication_id": "version:single-nucleus-analysis-of-human-white-adipose-tissue-reveals-adipocyte-subsets-with-distinct-metabolic-profiles--2025-09-16-biorxiv",
  "source_work_id": "work:single-nucleus-analysis-of-human-white-adipose-tissue-reveals-adipocyte-subsets-with-distinct-metabolic-profiles"
}
```

## Connections (1)

### Single-Nucleus Analysis of Human White Adipose Tissue Reveals Adipocyte Subsets with Distinct Metabolic Profiles

- ID: `version:single-nucleus-analysis-of-human-white-adipose-tissue-reveals-adipocyte-subsets-with-distinct-metabolic-profiles--2025-09-16-biorxiv`
- Kind: `publication`
- Direction: `incoming`
- URL: <https://sina.bio/publication/single-nucleus-analysis-of-human-white-adipose-tissue-reveals-adipocyte-201b7737>

This publication contains this grounded claim.

> Gene expression analysis utilized QuantStudio software (Applied Biosystems); relative mRNA concentrations normalized to the expression of the housekeeping gene were calculated using the ∆∆Ct method.

Source: 2025-09-16-biorxiv.pdf — <https://sina.bio/source/2025-09-16-biorxiv-pdf>