Long-read sequencing transcriptome quantification with lr-kallisto

Long-read sequencing platforms can support full-length isoform quantification, but isoform complexity, genetic variation, and long-read error profiles create computational challenges. Efficient methods are needed to accurately associate long reads with transcripts of origin across current long-read data types. lr-kallisto accurately and efficiently quantifies long-read RNA-seq libraries, supports bulk, single-cell, and single-nucleus data, benefits from exome capture, and uses less memory than compared long-read tools.